Part:BBa_K5038026
PT7-lacO-LIRA_input05-T7TE
Usage and Biology
LIRA_input05 is the target to be detected by LIRA_H05, it will bind to the loop at the top of LIRA_H05, and then change its conformation so that the ribosome could bind to the RBS.
(Figure 1. LIRA_input05 binds to LIRA_H05)
This composite part is used to express input05 in E.coli to test the function of H05.
Characterization
T7 promoter could be induced by IPTG, so the intracellularly concentration of LIRA_input05 produces a large difference between the two conditions of IPTG presence/absence.
Compared to BBa_K5038024, this plasmid has an additional lac operator downstream of T7 promoter, which is the binding site of LacI repression protein and could further prevent the transcription leakage.
This composite part is equipped by the following plasmids and co-transformed with the corresponding H05 plasmid. See BBa_K5038022 for details.
(Table 1. LIRA_input05 plasmids and the corresponding H05 plasmid)
| No. | LIRA_input05 plasmids | corresponding H05 plasmid |
|---|---|---|
| 1 | pCDFDuet-lacO-input05 | pET-15b-lacO-H05-linker-EGFP |
| 2 | pCOLADuet-lacO-input05 | pET-15b-lacO-H05-linker-EGFP |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |